维生素A对大鼠半乳糖胺诱导性肝炎发展的影响。

Effect on vitamin A on the development of galactosamine-induced hepatitis in rats.

作者信息

Büchsel R, Heissmeyer H, Lesch R, Stein U

出版信息

Pathol Res Pract. 1978 Sep;163(1):57-66. doi: 10.1016/s0344-0338(78)80110-1.

Abstract

In order to elucidate the role of lysosomes in experimental hepatitis caused by D-galactosamine in rats the activities of cathepsin A and D and acid carboxypeptidase were measured. Enzyme activities were assayed in liver homogenate, lysosomal supernatant fraction and lysosomal sediment fraction. Lysosomal enriched fractions were prepared according to De Duve. Vitamin A in high doses aggravates the morphological alterations observed in galactosamine treated rats. At the same time the labilization of lysosomes increases substantially. This effect was induced by doses of retinyl-palmitate that normally caused only an activation of Kupffer cells and no significant liberation of lysosomal peptidehydrolases. The activities of cathepsin A and D increased 2-fold in liver homogenate after combined treatment with galactosamine and vitamin A, whereas the activity of acid carboxypeptidase decreased markedly.

摘要

为了阐明溶酶体在大鼠D-半乳糖胺所致实验性肝炎中的作用，我们测定了组织蛋白酶A、D和酸性羧肽酶的活性。酶活性在肝脏匀浆、溶酶体上清液组分和溶酶体沉淀物组分中进行测定。根据德·迪夫的方法制备富含溶酶体的组分。高剂量维生素A会加重半乳糖胺处理大鼠所观察到的形态学改变。与此同时，溶酶体的不稳定性显著增加。这种效应是由正常情况下仅引起库普弗细胞激活且不会导致溶酶体肽水解酶显著释放的棕榈酸视黄酯剂量所诱导的。半乳糖胺与维生素A联合处理后，肝脏匀浆中组织蛋白酶A和D的活性增加了2倍，而酸性羧肽酶的活性则显著降低。