Cato A C, Sillar G M, Kioussis J, Burdon R H
Gene. 1981 Dec;16(1-3):27-34. doi: 10.1016/0378-1119(81)90058-5.
The method of differential in situ colony hybridisation and the technique of two-dimensional gel electrophoresis of polypeptides synthesized in vitro by mRNAs that hybridise specifically to recombinant plasmids bound to filter discs, have been used to isolate two cDNA HeLa heat-shock clones. Each of these two clones contains the plasmid pBR322 with a cDNA fragment of about 0.3 kb inserted into the PstI site. The two fragments hae different restriction maps. Whilst one of the recombinant plasmids, pHS2, contains a cDNA sequence that hybridises to mRNA coding for the HeLa heat-shock gamma-polypeptide, the other, pHS6, cross-hybridises with mRNAs that code for the HeLa heat-shock beta-, delta- and epsilon-polypeptides.
采用差异原位菌落杂交法以及对与结合在滤膜上的重组质粒特异性杂交的mRNA体外合成的多肽进行二维凝胶电泳技术，分离出了两个HeLa热休克cDNA克隆。这两个克隆中的每一个都含有质粒pBR322，在其PstI位点插入了一个约0.3 kb的cDNA片段。这两个片段具有不同的限制性图谱。其中一个重组质粒pHS2含有一个与编码HeLa热休克γ多肽的mRNA杂交的cDNA序列，另一个pHS6则与编码HeLa热休克β、δ和ε多肽的mRNA交叉杂交。
