Cheney C M, Rojko J L, Kociba G J, Wellman M L, Di Bartola S P, Rezanka L J, Forman L, Mathes L E
Department of Veterinary Pathobiology, Ohio State University, Columbus 43210.
In Vitro Cell Dev Biol. 1990 May;26(5):455-63. doi: 10.1007/BF02624087.
A lymphoma cell line (MCC) was derived from an abdominal mass from a 13-yr-old castrated male cat. The cells resemble natural killer precursor cells, have membrane-bound granules, and are positive for chloroacetate esterase, alpha-naphthyl butyrate esterase, and tartrate-resistant acid phosphatase activities. The MCC cells are negative for rearranged feline T-cell receptor genes, negative for feline T-cytotoxic antigen, Ia, and surface mu, tau, and lambda chains and do not form E-rosettes. The MCC cell line is negative for the feline leukemia virus (FeLV); e.g., negative for exogenous FeLV (exU3) sequences, negative for cytoplasmic and surface FeLV major core protein of 27,000 daltons (p27) by indirect immunofluorescence assay, negative for helper FeLV by clone 81 assay, and negative for release of soluble FeLV p27 by enzyme-linked immunosorbent assay. Electron microscopy reveals budding type C retrovirus particles and MCC cells react with anti-RD-114 (anti-endogenous feline retrovirus) reference serum. After in vitro infection, MCC replicate FeLV readily, but replication is noncytopathic.
一株淋巴瘤细胞系（MCC）源自一只13岁去势雄性猫的腹部肿块。这些细胞类似于自然杀伤前体细胞，具有膜结合颗粒，且氯乙酸酯酶、α-萘丁酸酯酶和抗酒石酸酸性磷酸酶活性呈阳性。MCC细胞的猫T细胞受体基因重排呈阴性，猫T细胞毒性抗原、Ia以及表面μ、τ和λ链呈阴性，且不形成E花环。MCC细胞系对猫白血病病毒（FeLV）呈阴性；例如，对外源FeLV（exU3）序列呈阴性，通过间接免疫荧光法检测27000道尔顿的细胞质和表面FeLV主要核心蛋白（p27）呈阴性，通过克隆81法检测辅助性FeLV呈阴性，通过酶联免疫吸附测定法检测可溶性FeLV p27的释放呈阴性。电子显微镜显示出芽型C逆转录病毒颗粒，且MCC细胞与抗RD - 114（抗内源性猫逆转录病毒）参考血清发生反应。体外感染后，MCC很容易复制FeLV，但复制不具有细胞病变效应。
