人淋巴细胞在无饲养层的琼脂糖中具有高克隆效率。
High cloning efficiency of human lymphoid cells in agarose without feeder layers.
作者信息
Ayres K N
出版信息
J Natl Cancer Inst. 1982 Jun;68(6):919-23.
PMID:7045487
Abstract
A protocol has been developed that consistently gives high cloning efficiencies for human lymphoid cell lines (lymphoblastoid and lymphoma) in agarose without the use of feeder layers. This procedure utilizes a cloning medium that contains horse serum, alpha-ketoglutarate or oxalacetate, and high levels of glutamine.
摘要
已开发出一种方案，该方案在不使用饲养层的情况下，能始终如一地在琼脂糖中为人类淋巴样细胞系（淋巴母细胞样和淋巴瘤细胞系）提供高克隆效率。此程序使用一种含有马血清、α-酮戊二酸或草酰乙酸以及高水平谷氨酰胺的克隆培养基。
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