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人淋巴细胞在无饲养层的琼脂糖中具有高克隆效率。

High cloning efficiency of human lymphoid cells in agarose without feeder layers.

作者信息

Ayres K N

出版信息

J Natl Cancer Inst. 1982 Jun;68(6):919-23.

PMID:7045487
Abstract

A protocol has been developed that consistently gives high cloning efficiencies for human lymphoid cell lines (lymphoblastoid and lymphoma) in agarose without the use of feeder layers. This procedure utilizes a cloning medium that contains horse serum, alpha-ketoglutarate or oxalacetate, and high levels of glutamine.

摘要

已开发出一种方案，该方案在不使用饲养层的情况下，能始终如一地在琼脂糖中为人类淋巴样细胞系（淋巴母细胞样和淋巴瘤细胞系）提供高克隆效率。此程序使用一种含有马血清、α-酮戊二酸或草酰乙酸以及高水平谷氨酰胺的克隆培养基。

相似文献

1
High cloning efficiency of human lymphoid cells in agarose without feeder layers.人淋巴细胞在无饲养层的琼脂糖中具有高克隆效率。
J Natl Cancer Inst. 1982 Jun;68(6):919-23.
2
Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines.人恶性淋巴瘤细胞系建立与克隆的饲养层及营养需求
Cancer Res. 1979 May;39(5):1748-59.
3
A new cloning method for antibody-forming lymphoblastoid cells. Increase in cloning efficiency by inclusion of human fibroblasts into semisolid agarose growth layer.
J Immunol Methods. 1986 Nov 20;94(1-2):201-8. doi: 10.1016/0022-1759(86)90234-6.
4
Soft-agar cloning of cells from patients with lymphoma.淋巴瘤患者细胞的软琼脂克隆
Prog Clin Biol Res. 1980;48:43-52.
5
Senescent fibroblasts as feeder cells for lymphoid cell cloning.
Anal Biochem. 1996 May 1;236(2):250-4. doi: 10.1006/abio.1996.0163.
6
Improvement of EBV transformation and cloning efficiency of human B cells using culture supernatants from lymphoblastoid cell lines.利用淋巴母细胞系的培养上清液提高人B细胞的EBV转化和克隆效率。
Hum Antibodies Hybridomas. 1993 Apr;4(2):66-73.
7
Serum free cultivation of lymphoid cells for virus and interferon production.用于病毒和干扰素生产的淋巴细胞无血清培养。
Dev Biol Stand. 1985;60:349-54.
8
Effects of feeder layers made of human, mouse, hamster, and rat cells on the cloning efficiency of transformed human cells.人、小鼠、仓鼠和大鼠细胞制成的饲养层对转化人细胞克隆效率的影响。
In Vitro. 1982 May;18(5):469-75. doi: 10.1007/BF02796475.
9
Antiproliferative lymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double layer soft agarose clonogenic assay.通过改良的双层软琼脂克隆形成试验检测人外周血淋巴细胞和淋巴结淋巴细胞产生的抗增殖淋巴因子。
Lymphokine Cytokine Res. 1994 Feb;13(1):55-62.
10
High-efficiency human B-cell cloning using hygromycin B-resistant feeder cells.
Biotechniques. 1997 May;22(5):814-6, 820, 822. doi: 10.2144/97225bm05.

引用本文的文献

1
Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridization.通过细胞杂交研究人淋巴母细胞中DNA O6-甲基鸟嘌呤去除能力的调控。
Mol Cell Biol. 1982 Aug;2(8):904-13. doi: 10.1128/mcb.2.8.904-913.1982.
2
Hormone supplemented media for cloning human breast cancer: increased colony formation without alteration of chemosensitivity.用于克隆人乳腺癌的激素补充培养基：增加集落形成而不改变化学敏感性。
Br J Cancer. 1983 Nov;48(5):683-8. doi: 10.1038/bjc.1983.250.
3
Heterogeneous origin of established non-T,non-B cell lines from two adolescent patients with acute lymphoblastic leukemia.
两名急性淋巴细胞白血病青少年患者中已建立的非T、非B细胞系的异质性起源。
Jpn J Cancer Res. 1988 Jan;79(1):59-68. doi: 10.1111/j.1349-7006.1988.tb00011.x.