用于病毒和干扰素生产的淋巴细胞无血清培养。
Serum free cultivation of lymphoid cells for virus and interferon production.
作者信息
Bjare U, Räbb I
出版信息
Dev Biol Stand. 1985;60:349-54.
PMID:2995174
Abstract
Minor modifications of standard media have given a defined serum free medium for the production of polio virus and measles virus in lymphoblastoid cells and of Namalva interferon in lymphoma cells. By usage of slightly modified standard roller equipment in the order of 2 X 10 12TCID-50 of polio virus and 4 X 10(9) IU of interferon can be produced on one roller rack. The produced poliovirus does comply with requirements for inactivated poliovaccine.
摘要
对标准培养基进行微小改良后，得到了一种特定的无血清培养基，用于在淋巴母细胞样细胞中生产脊髓灰质炎病毒和麻疹病毒，以及在淋巴瘤细胞中生产纳马夸兰干扰素。通过使用稍加改良的标准滚瓶设备，在一个滚瓶架上可生产约2×10¹²组织培养感染剂量50（TCID-50）的脊髓灰质炎病毒和4×10⁹国际单位（IU）的干扰素。所生产的脊髓灰质炎病毒符合灭活脊髓灰质炎疫苗的要求。
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