Bogdarin Iu A
Probl Endokrinol (Mosk). 1978 Sep-Oct;24(5):48-52.
The author suggests a method of blood testosterone level determination on gas chromatograph "Tsvet-104" with a constant recombination rate detector, without any use of radioactive isotopes, testosterone derivatives. The method consists in thorough preliminary extraction of endogenous testosterone from the blood, its purification in thin layers on silicalgel, and conversion into a fluoroacetoderivative with a subsequent gaseochromatographic separation on chromatone N-AW-DMCS (60--80 mesh) with a low (5%) SE-30 phase content at high temeprature regimens of gas chromatograph "Tsvet-104" with a high resolving power of its selective detector.
作者提出了一种在配备恒定复合率检测器的“Tsvet - 104”气相色谱仪上测定血液睾酮水平的方法，该方法不使用任何放射性同位素和睾酮衍生物。该方法包括从血液中彻底预先提取内源性睾酮，在硅胶薄层上进行纯化，然后转化为氟代乙酰衍生物，随后在“Tsvet - 104”气相色谱仪的高温条件下，在具有低（5%）SE - 30相含量的Chromatone N - AW - DMCS（60 - 80目）上进行气相色谱分离，其选择性检测器具有高分辨能力。
