整合了大肠杆菌色氨酸操纵子附近区域的沙门氏菌紫外线可诱变杂种。
UV-mutable hybrids of Salmonella incorporating Escherichia coli region adjacent to tryptophan operon.
作者信息
Skavronskaya A G, Stepanova N F, Andreeva I V
出版信息
Mol Gen Genet. 1982;185(2):315-8. doi: 10.1007/BF00330804.
PMID:7045586
Abstract
Salmonella typhimurium recombinants have been constructed which have acquired UV-induced mutability. Such recombinants were obtained after transfer of plasmid F'126 from E. coli. UV-mutability was acquired by UV-stable Salmonella as a nonselective marker after selection of trp+ marker in 2.5% of the cases. Possible deficiency in UV-stable Salmonella of the umuC gene is discussed.
摘要
已经构建出了获得紫外线诱导突变能力的鼠伤寒沙门氏菌重组体。这种重组体是在从大肠杆菌转移质粒F'126之后获得的。在2.5%的情况下，通过选择色氨酸+标记，紫外线稳定的沙门氏菌获得了紫外线诱导突变能力作为非选择性标记。文中讨论了umuC基因在紫外线稳定的沙门氏菌中可能存在的缺陷。
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