Diaz R, Barnsley P, Pritchard R H
Mol Gen Genet. 1979 Sep;175(2):151-7. doi: 10.1007/BF00425531.
A segment of DNA located in the region of the E. coli K12 chromosome previously identified by the Rac phenotype can function as a self-replicating plasmid. Evidence is presented that this plasmid, the oriJ plasmid, contains the origin of replication of a defective prophage postulated to be located in this chromosomal region by Low (1973). The plasmid can only be maintained in strains in which this postulated prophage has been deleted. In strains which possess the prophage selection for plasmid maintenance permits the isolation of clones containing new deletions which we postulate are the result of prophage excision.
位于大肠杆菌K12染色体上先前由Rac表型鉴定区域的一段DNA可作为自我复制质粒发挥作用。有证据表明，这种oriJ质粒包含一种缺陷原噬菌体的复制起点，Low（1973年）推测该原噬菌体位于这个染色体区域。该质粒只能在已删除这种推测原噬菌体的菌株中维持。在拥有该原噬菌体的菌株中，对质粒维持的选择允许分离出含有新缺失的克隆，我们推测这是原噬菌体切除的结果。
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