Ohe T
Mutat Res. 1982 May;101(3):175-87. doi: 10.1016/0165-1218(82)90151-3.
Nitrogenous compounds such as guanidine, ureide, secondary amines and polyamines were pyrolysed at 300, 400, 500 and 600 degrees C for 3 min, and the mutagenic activities of the pyrolysates were assayed on Salmonella typhimurium TA98 and TA100 with or without metabolic activation by S9 mix. Among 21 pyrolysates tested, 14, from methylguanidine, agmatine, dihydrouracil, dimethylamine, diethylamine, trimethylamine, triethylamine, pyrrolidine, morpholine, sarcosine, piperazine, piperidine, spermine and spermidine, showed mutagenic activity. In the presence of S9 mix, the mutagenic activity began to appear from the pyrolysate at 400 degrees C, and the pyrolysate at 600 degrees C showed the highest mutagenic activity except that from methylguanidine. The mutagenic activity formed by pyrolysis was more active on TA98 than TA100. In the absence of S9 mix, only 3 pyrolysates - from dimethylamine, diethylamine and pyrrolidine - showed slight mutagenic activity toward TA100. The highest mutagenic activity was observed with the pyrolysate from spermine, followed by those from piperidine, spermidine, piperazine and triethylamine. Some nitrogenous compounds showed slight mutagenic activity after pyrolysis at 300 degrees C for 20 min, although none of the compounds tested showed any mutagenic activity after pyrolysis at 300 degrees C for 3 min.
胍、脲、仲胺和多胺等含氮化合物在300、400、500和600℃下热解3分钟，热解产物的致突变活性在有或无S9混合物代谢活化的情况下，在鼠伤寒沙门氏菌TA98和TA100上进行测定。在测试的21种热解产物中，来自甲基胍、胍丁胺、二氢尿嘧啶、二甲胺、二乙胺、三甲胺、三乙胺、吡咯烷、吗啉、肌氨酸、哌嗪、哌啶、精胺和亚精胺的14种热解产物表现出致突变活性。在有S9混合物存在的情况下，400℃热解产物开始出现致突变活性，600℃热解产物除甲基胍外致突变活性最高。热解形成的致突变活性对TA98的作用比对TA100更明显。在没有S9混合物的情况下，只有来自二甲胺、二乙胺和吡咯烷的3种热解产物对TA100表现出轻微的致突变活性。观察到精胺热解产物的致突变活性最高，其次是哌啶、亚精胺、哌嗪和三乙胺的热解产物。一些含氮化合物在300℃热解20分钟后表现出轻微的致突变活性，尽管所测试的化合物在300℃热解3分钟后均未表现出任何致突变活性。
