兔晶状体纤维细胞中晶状体蛋白的生物合成。II. 培养基的影响。
Biosynthesis of crystallin by rabbit lens fiber cells. II. Effect of culture medium.
作者信息
Bagchi M
出版信息
Ophthalmic Res. 1982;14(2):148-52. doi: 10.1159/000265186.
PMID:7045763
Abstract
Freshly isolated lenses incubated in either M-199 alone or supplemented with serum or insulin were measured for alpha- and gamma-crystallin synthesis. It was found that synthesis of these crystallins were not altered by addition of either serum of insulin in the culture medium. The alpha-crystallin synthesis showed decreased synthetic activity after 1 day of culture, whereas gamma-crystallin synthesis maintained a comparatively similar rate of synthesis for 2 days.
摘要
对新鲜分离的晶状体进行测量，这些晶状体分别在单独的M-199培养基中培养，或添加血清或胰岛素的M-199培养基中培养，以检测α-晶状体蛋白和γ-晶状体蛋白的合成情况。结果发现，在培养基中添加血清或胰岛素并不会改变这些晶状体蛋白的合成。α-晶状体蛋白的合成在培养1天后合成活性降低，而γ-晶状体蛋白的合成在2天内保持相对相似的合成速率。
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