大肠杆菌RNA聚合酶与T7 DNA的激光交联
Laser crosslinking of E. coli RNA polymerase and T7 DNA.
作者信息
Harrison C A, Turner D H, Hinkle D C
出版信息
Nucleic Acids Res. 1982 Apr 10;10(7):2399-414. doi: 10.1093/nar/10.7.2399.
Abstract
The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration, and is a linear function of laser intensity. The protein subunits crosslinked to DNA are beta, beta' and sigma.
摘要
报道了使用激光激发将蛋白质与核酸进行首次光化学交联。在所研究的条件下，一个248nm的120mJ、20ns的单脉冲使约10%结合的大肠杆菌RNA聚合酶与T7 DNA发生交联。交联产率取决于巯基乙醇浓度，并且是激光强度的线性函数。与DNA交联的蛋白质亚基是β、β'和σ。
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