Latt S A
J Histochem Cytochem. 1977 Jul;25(7):913-26. doi: 10.1177/25.7.70459.
Fluorometric detection of the biosynthetic incorporation of 5-bromodeoxyuridine (BrdU) into deoxyribonucleic acid has permitted cytologic studies of chromosome structure, replication, and repair. Some of these phenomena, previously detected using BrdU-dye techniques on fixed microscopic preparations, should be particularly amenable to analogous experimentation in fluorescence flow systems. Problems involved in interfacing BrdU-dye methodology with flow fluorometry are discussed. The effects of certain chemical modifications of bisbenzimidazole dyes on their spectroscopic properties and potential use for detecting BrdU incorporation into unfixed cells are described. Data on the use and energy transfer characteristics of a pair of deoxyribonucleic acid-binding dyes (33258 Hoechst and ethidium bromide) capable of simultaneously providing information about BrdU substitution and total deoxyribonucleic acid content are presented.
通过荧光法检测5-溴脱氧尿苷（BrdU）在脱氧核糖核酸中的生物合成掺入，已能够对染色体结构、复制及修复进行细胞学研究。其中一些现象，此前在固定显微镜标本上使用BrdU-染料技术检测到，在荧光流式细胞系统中应特别适合进行类似实验。本文讨论了将BrdU-染料方法与流式荧光测定法相结合所涉及的问题。描述了双苯并咪唑染料的某些化学修饰对其光谱性质的影响以及在检测BrdU掺入未固定细胞中的潜在用途。给出了一对能够同时提供有关BrdU取代和总脱氧核糖核酸含量信息的脱氧核糖核酸结合染料（Hoechst 33258和溴化乙锭）的使用及能量转移特性数据。
