Latt S A, Stetten G
J Histochem Cytochem. 1976 Jan;24(1):24-33. doi: 10.1177/24.1.943439.
Absorption, fluroescence and circular dichroism measrements on 33258 Hoechst-deoxyribonucleic acid (DNA) complexes are consistent with the existence of two types of dye-binding interactions. One type, which persists at elevated solution ionic strength, is highly specific for adenine-thymine-rich DNA. Dye bound under this condition exhibits efficient fluorescence and strong optical activity. A less specific, largely electrostatic interaction is associated with less intense fluorescence and weaker optical activity. The fluorescence of 33258 Hoechst and several other bisbenzimidazole dyes is less when bound to poly(deoxyadenylate-5-bromodeoxyuridylate) than when bound to poly(deoxyadenlyate-deoxythymidylate). Quenching of 33258 Hoechst fluorescence can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. This property of 33258 Hoechst should allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis, providing a bridge between biochemical and cytologic analyses of processes related to DNA replication.
对33258 Hoechst - 脱氧核糖核酸（DNA）复合物的吸收、荧光和圆二色性测量结果与两种类型的染料结合相互作用的存在相一致。其中一种类型在溶液离子强度升高时仍然存在，对富含腺嘌呤 - 胸腺嘧啶的DNA具有高度特异性。在这种条件下结合的染料表现出高效的荧光和强烈的光学活性。一种特异性较低、主要是静电作用的相互作用则与较弱的荧光和较弱的光学活性相关。33258 Hoechst和其他几种双苯并咪唑染料与聚（脱氧腺苷酸 - 5 - 溴脱氧尿苷酸）结合时的荧光比与聚（脱氧腺苷酸 - 脱氧胸苷酸）结合时弱。3'258 Hoechst荧光的猝灭也可用于检测5 - 溴脱氧尿苷在活细胞DNA中的生物合成掺入。33258 Hoechst的这一特性应能根据DNA合成的程度进行荧光激活细胞和染色体分选，为与DNA复制相关过程的生化分析和细胞学分析之间架起一座桥梁。
