Butler J E, Klobasa F, Werhahn E
Vet Immunol Immunopathol. 1981 Feb;2(1):53-65. doi: 10.1016/0165-2427(81)90038-6.
The localization of immunoglobulin IgG, IgM and IgA in tissue sections prepared from the ileum of neonatal and adult swine were compared. Eighty percent of the immunoglobulin-containing lymphoid cells in the lamina propria of conventional adult German Landrasse swine were IgA-positive with lower numbers of IgM cells and occasionally an IgG cell. Anti-mu and alpha-chain reagents also stained the cytoplasm of the crypt epithelial cells. By comparison to these adult control tissues, the ileum of unsuckled neonates contained no immunoglobulins although after the ingestion of colostrum, the entire cytoplasm of the villus epithelial cells stained intensely when tested for IgG with only faint staining for IgM and IgA. On the other hand, IgA and IgM were readily localized on what appears to be only the apical border of the crypt epithelial cells but in contrast to the adult, the cytoplasm of these cells was unlabelled. IgG was absent from the crypt region. We interprete these findings to indicate an important, selective role for the villus epithelium in the absorption into the neonatal circulation of colostral IgG and probably IgA and IgM, and a specialized role for the crypt epithelium in adsorbing colostral IgA and IgM; possibly by complexing with mucin-bound secretory component.
对新生猪和成年猪回肠制备的组织切片中免疫球蛋白IgG、IgM和IgA的定位进行了比较。在传统成年德国长白猪固有层中，80%含免疫球蛋白的淋巴细胞为IgA阳性，IgM细胞数量较少，偶尔有IgG细胞。抗μ链和α链试剂也使隐窝上皮细胞的细胞质着色。与这些成年对照组织相比，未吮乳的新生猪回肠不含免疫球蛋白，不过在摄入初乳后，用IgG检测时，绒毛上皮细胞的整个细胞质都强烈染色，而IgM和IgA染色较淡。另一方面，IgA和IgM很容易定位在似乎仅为隐窝上皮细胞的顶端边界，但与成年猪不同的是，这些细胞的细胞质未被标记。隐窝区域没有IgG。我们将这些发现解释为，绒毛上皮在初乳IgG以及可能的IgA和IgM吸收进入新生猪循环中起重要的选择性作用，而隐窝上皮在吸附初乳IgA和IgM中起特殊作用；可能是通过与黏蛋白结合的分泌成分形成复合物来实现。
