Polezhaev L V
Zh Vopr Neirokhir Im N N Burdenko. 1982 Mar-Apr(2):53-7.
A destruction method was elaborated in dog experiments which allowed bone defects in the skull to be repaired with regenerating bone. The bone is ground to fillings, moistened with the recipient's blood, and implanted in the bone defect in the skull onto the dura mater which is necessary for bone regeneration. The bone fillings dissolve within 7 days, their cells perish and the substances released from them induce osteogenesis in the cells of the immature connective tissue. Fresh bone filings may be used for auto-, allo- and heteroplasty. They preserve their osteo-inducing property after freezing at--78 degrees C and are inactivated after being autoclaved and treated with 0.5% formaldehyde solution or diocide solution. Lyophilization reduces the osteo-inducing property of bone fillings.
在犬实验中精心设计了一种破坏方法，该方法能使颅骨骨缺损用再生骨修复。将骨研磨成填充物，用受体血液浸湿，然后植入颅骨骨缺损处的硬脑膜上，硬脑膜对骨再生是必需的。骨填充物在7天内溶解，其细胞死亡，从中释放的物质诱导未成熟结缔组织细胞发生骨生成。新鲜骨屑可用于自体移植、同种异体移植和异种移植。它们在-78℃冷冻后仍保留其骨诱导特性，在高压灭菌并用0.5%甲醛溶液或二氧化氯溶液处理后失活。冻干会降低骨填充物的骨诱导特性。
