James K, Baum L L, Vetter M L, Gewurz H
Ann N Y Acad Sci. 1982;389:274-85. doi: 10.1111/j.1749-6632.1982.tb22143.x.
The interactions between CRP and peripheral blood lymphocytes were investigated. CRP, in the presence of an appropriate ligand, bound saturably to a small percentage of normal PBL. The characteristics and optimal conditions for this binding were defined using several different assay systems. CRP was found to bind preferentially to cells with the IgG FcR. Binding was increased in the presence of acute phase sera, and higher numbers of cells binding CRP were observed in acute phase individuals. CRP and CRP-CPS had minimal effects upon lymphocyte responsiveness in vitro, although enhancing effects on MLC and CMC reactions, and a slight blastogenic effect, were observed. CRP antigenicity was detected on a small percentage of PBL, and treatment of PBL with anti-CRP and complement led to loss of NK reactivity, suggesting a possible association of CRP with this function. The functional expression of binding of CRP complexes, the relationship of the CRP-binding site to surface CRP antigenicity and the FcR, and the role of these factors in lymphocyte functions such as NK reactivity and recognition, are yet to be determined.
研究了CRP与外周血淋巴细胞之间的相互作用。在存在适当配体的情况下，CRP可饱和结合一小部分正常外周血淋巴细胞（PBL）。使用几种不同的检测系统确定了这种结合的特征和最佳条件。发现CRP优先结合具有IgG FcR的细胞。在急性期血清存在下结合增加，并且在急性期个体中观察到更多结合CRP的细胞。CRP和CRP-CPS对体外淋巴细胞反应性的影响最小，尽管观察到对混合淋巴细胞培养（MLC）和细胞介导的细胞毒作用（CMC）反应有增强作用以及轻微的促有丝分裂作用。在一小部分PBL上检测到CRP抗原性，用抗CRP和补体处理PBL导致自然杀伤（NK）反应性丧失，提示CRP与该功能可能存在关联。CRP复合物结合的功能表达、CRP结合位点与表面CRP抗原性和FcR的关系以及这些因素在淋巴细胞功能如NK反应性和识别中的作用尚待确定。
