Giclas P C, Manthei U, Strunk R C
Am J Pathol. 1985 Jul;120(1):146-56.
Concentrations of five serum proteins, C3, C5, ceruloplasmin, C-reactive protein, and albumin, have been measured during the acute phase response in rabbits with turpentine-induced pleurisy. C-reactive protein concentrations in the circulation rose abruptly between 12 and 36 hours to a level greater than 50 times the pretreatment concentration, then returned to undetectable amounts by 96 hours. C3 and ceruloplasmin both showed some increase in concentration by 12 hours and reached their maximum concentrations of two to three times the baseline levels 48-72 hours after the turpentine treatment. Concentrations were still elevated at 120 hours, after which time they gradually returned to normal. C5 and albumin concentrations in the turpentine-treated rabbits did not differ from the baseline concentrations. The same five proteins were measured in the inflammatory exudate. C-reactive protein was not detectable at any of the time points. C3, C5, ceruloplasmin, and albumin were present in normal pleural fluid at roughly half their serum concentrations. The activities of C3, C5, and ceruloplasmin were low in the early exudate, but C3 and C5 activity rose relative to their concentrations in the later samples of pleural fluid. The specific activities of C3 and C5 were higher in the pleural fluid at 72 hours than in plasma, while that of ceruloplasmin remained less in the pleural fluid than in plasma throughout the experiment. The involvement of these proteins and their relation to the inflammatory response are discussed.
在松节油诱导胸膜炎的家兔急性期反应过程中，测定了5种血清蛋白（C3、C5、铜蓝蛋白、C反应蛋白和白蛋白）的浓度。循环中的C反应蛋白浓度在12至36小时之间急剧上升，升至预处理浓度的50倍以上，然后在96小时时降至检测不到的水平。C3和铜蓝蛋白在12小时时浓度均有所升高，并在松节油处理后48 - 72小时达到其最高浓度，为基线水平的两到三倍。在120小时时浓度仍升高，此后逐渐恢复正常。松节油处理的家兔中C5和白蛋白浓度与基线浓度无差异。在炎性渗出物中也测定了同样的5种蛋白。在任何时间点均未检测到C反应蛋白。C3、C5、铜蓝蛋白和白蛋白在正常胸液中的浓度约为血清浓度的一半。早期渗出物中C3、C5和铜蓝蛋白的活性较低，但在后来的胸液样本中，C3和C5的活性相对于其浓度有所升高。在72小时时，胸液中C3和C5的比活性高于血浆，而在整个实验过程中，胸液中铜蓝蛋白的比活性仍低于血浆。讨论了这些蛋白的参与情况及其与炎症反应的关系。
