Myler P, Chiu S, Upcroft J, Castelino D, Kidson C
Aust J Exp Biol Med Sci. 1982 Feb;60(Pt 1):91-6. doi: 10.1038/icb.1982.7.
Proteins from synchronized continuous cultures of Plasmodium falciparum were biosynthetically labelled with [35S]methionine at ring, trophozoite, and schizont stages. Several proteins appeared to be synthesized predominantly at one stage only, especially the schizont stage. Immunoprecipitation using inhibitory human sera revealed that a variety of proteins, from all stages of parasite growth, were recognized. More proteins, especially those with high molecular weight, were precipitated from the schizont stage than from ring and trophozoite stages, but two major proteins (MW or approximately 70,000 daltons and or approximately 45,000 daltons) were recognized in all three stages.
恶性疟原虫同步连续培养物中的蛋白质在环状体、滋养体和裂殖体阶段用[35S]甲硫氨酸进行生物合成标记。几种蛋白质似乎仅在一个阶段大量合成，尤其是裂殖体阶段。使用抑制性人血清进行免疫沉淀显示，寄生虫生长各阶段的多种蛋白质都能被识别。与环状体和滋养体阶段相比，从裂殖体阶段沉淀出的蛋白质更多，尤其是那些高分子量的蛋白质，但在所有三个阶段都能识别出两种主要蛋白质（分子量约为70,000道尔顿和约45,000道尔顿）。
