Brown G V, Anders R F, Stace J D, Alpers M P, Mitchell G F
Parasite Immunol. 1981 Winter;3(4):283-98. doi: 10.1111/j.1365-3024.1981.tb00407.x.
The human serum antibody response to Plasmodium falciparum infection in Papua New Guinea has been studied by electrophoretic analysis of immunoprecipitated biosynthetically-labelled malaria proteins from three different isolates maintained in long-term in vitro culture. Differences in protein antigenic composition in different lines have been described and simplified by examination of antigens recognized only by hyperimmune serum. An in vitro assay has been used to screen various human sera containing antimalarial antibody for their ability to inhibit parasite growth and the immunoprecipitation profiles of non-inhibitory sera have been compared with those of a hyperimmune serum pool. In the discussion, emphasis is placed on the value of immunoprecipitation analyses using clinically-defined sera with known in vitro function in the identification of antigens which may be responsible for the induction of host-protective immunity.
通过对长期体外培养的三种不同疟原虫分离株进行生物合成标记的疟疾蛋白免疫沉淀产物的电泳分析，研究了巴布亚新几内亚人体对恶性疟原虫感染的血清抗体反应。通过检测仅被超免疫血清识别的抗原，描述并简化了不同株系中蛋白质抗原组成的差异。已使用体外试验筛选含有抗疟抗体的各种人体血清抑制寄生虫生长的能力，并将非抑制性血清的免疫沉淀图谱与超免疫血清库的图谱进行了比较。在讨论中，重点强调了使用具有已知体外功能的临床定义血清进行免疫沉淀分析在鉴定可能诱导宿主保护性免疫的抗原方面的价值。
