泌乳大鼠乳腺和肝脏中鸟氨酸脱羧酶及S-腺苷甲硫氨酸脱羧酶活性的调节。饥饿、催乳素和胰岛素缺乏的影响。

Regulation of the activity of ornithine decarboxylase and S-adenosylmethionine decarboxylase in mammary gland and liver of lactating rats. Effects of starvation, prolactin and insulin deficiency.

作者信息

Brosnan M E, Ilic V, Williamson D H

出版信息

Biochem J. 1982 Mar 15;202(3):693-8. doi: 10.1042/bj2020693.

DOI:10.1042/bj2020693

PMID:7046736

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158164/

Abstract

Starvation caused a marked decrease in the activity of ornithine decarboxylase in mammary gland, together with a lesser decrease in the activity of S-adenosylmethionine decarboxylase and a marked fall in milk production. Liver ornithine decarboxylase and S-adenosylmethionine decarboxylase activities were unaffected. 2. Refeeding for 2.5 h was without effect on ornithine decarboxylase in mammary gland, but it returned the S-adenosylmethionine decarboxylase activity in mammary gland to control values and elevated both ornithine decarboxylase and S-adenosylmethionine decarboxylase in liver. 3. Refeeding for 5 h returned the activity of ornithine decarboxylase in mammary gland to fed-state values and resulted in further increases in S-adenosylmethionine decarboxylase in mammary gland and liver and in ornithine decarboxylase in liver. 4. Prolactin deficiency in fed rats resulted in decreased milk production and decreased activity of ornithine decarboxylase in mammary gland. The increase in ornithine decarboxylase activity normally seen after refeeding starved rats for 5 h was completely blocked by prolactin deficiency. 5. In fed rats, injection of streptozotocin 2.5 h before death caused a decrease in the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase in mammary gland, which could be reversed by simultaneous injection of insulin. Insulin deficiency also prevented the increase in S-adenosylmethionine decarboxylase in liver and mammary gland normally observed after refeeding starved rats for 2.5 h.

摘要

饥饿导致乳腺中鸟氨酸脱羧酶的活性显著降低，同时S-腺苷甲硫氨酸脱羧酶的活性降低幅度较小，且产奶量显著下降。肝脏中鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶的活性未受影响。2. 再喂食2.5小时对乳腺中的鸟氨酸脱羧酶没有影响，但它使乳腺中S-腺苷甲硫氨酸脱羧酶的活性恢复到对照值，并提高了肝脏中鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶的活性。3. 再喂食5小时使乳腺中鸟氨酸脱羧酶的活性恢复到进食状态值，并导致乳腺和肝脏中S-腺苷甲硫氨酸脱羧酶以及肝脏中鸟氨酸脱羧酶的进一步增加。4. 喂食大鼠催乳素缺乏导致产奶量减少和乳腺中鸟氨酸脱羧酶的活性降低。饥饿大鼠再喂食5小时后通常出现的鸟氨酸脱羧酶活性增加被催乳素缺乏完全阻断。5. 在喂食大鼠中，死亡前2.5小时注射链脲佐菌素导致乳腺中鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶的活性降低，同时注射胰岛素可使其逆转。胰岛素缺乏也阻止了饥饿大鼠再喂食2.5小时后通常观察到的肝脏和乳腺中S-腺苷甲硫氨酸脱羧酶的增加。