用于多胺分析的大鼠乳腺上皮细胞的分离
Isolation of rat mammary epithelial cells for polyamine analysis.
作者信息
Minocha R, Thompson H J, Meeker L D, Herbst E J
出版信息
Biochem J. 1984 Nov 1;223(3):929-32. doi: 10.1042/bj2230929.
Abstract
Mammary epithelial cells were isolated from either abdominal-inguinal glands or mammary tumours of rats, after enzymic digestion of the tissues, and were analysed for polyamine content. Optimum conditions were developed for the isolation of cells in sufficient yield for the analysis of polyamines from 1 g of mammary gland or 0.5 g of tumour tissue. Complete recoveries of the polyamines in the tissues were achieved in the isolated epithelial cells.
摘要
在对大鼠的腹部腹股沟腺体或乳腺肿瘤组织进行酶消化后，分离出乳腺上皮细胞，并对其多胺含量进行分析。已确定了最佳条件，以便从1克乳腺组织或0.5克肿瘤组织中分离出足够数量的细胞用于多胺分析。在分离出的上皮细胞中实现了组织中多胺的完全回收。
相似文献
1
Isolation of rat mammary epithelial cells for polyamine analysis.用于多胺分析的大鼠乳腺上皮细胞的分离
Biochem J. 1984 Nov 1;223(3):929-32. doi: 10.1042/bj2230929.
2
Immunolocalization of the human basal epithelial marker monoclonal antibody 312C8-1 in normal tissue and mammary tumours of rodents.人类基底上皮标志物单克隆抗体312C8 - 1在啮齿动物正常组织和乳腺肿瘤中的免疫定位
Virchows Arch A Pathol Anat Histopathol. 1989;415(6):533-8. doi: 10.1007/BF00718646.
3
Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats.饥饿和再喂养对泌乳大鼠乳腺中多胺浓度及鸟氨酸脱羧酶抗酶的影响。
Biochem J. 1983 Apr 15;212(1):149-53. doi: 10.1042/bj2120149.
4
Immunocytochemical identification of myoepithelial cells in normal and neoplastic rat mammary glands with the lectins Griffonia simplicifolia-1 and pokeweed mitogen.用凝集素单叶豆凝集素-1和商陆有丝分裂原对正常和肿瘤大鼠乳腺中的肌上皮细胞进行免疫细胞化学鉴定。
J Histochem Cytochem. 1990 Nov;38(11):1633-45. doi: 10.1177/38.11.2170504.
5
Cultured proliferating rat mammary epithelial cells.培养的增殖型大鼠乳腺上皮细胞。
In Vitro Cell Dev Biol. 1991 Sep;27A(9):749-54. doi: 10.1007/BF02633221.
6
Dispersed mammary gland epithelial cells. I. Isolation and separation procedures.分散的乳腺上皮细胞。I. 分离与分选程序。
J Cell Biol. 1977 Feb;72(2):390-405. doi: 10.1083/jcb.72.2.390.
7
Isolation and characterization of epithelial stem-cell cell lines from the rat mammary gland.从大鼠乳腺中分离并鉴定上皮干细胞系
Br J Cancer. 1980 Apr;41(4):666-8. doi: 10.1038/bjc.1980.119.
8
Transcapillary passage of albumin in mammary tumours and in normal lactating mammary glands of the rat.白蛋白在大鼠乳腺肿瘤及正常泌乳乳腺中的跨毛细血管转运
Acta Physiol Scand. 1984 Dec;122(4):497-505. doi: 10.1111/j.1748-1716.1984.tb07538.x.
9
Isolation, culture and analysis of mouse mammary epithelial cells.小鼠乳腺上皮细胞的分离、培养与分析。
Methods Mol Biol. 2010;633:139-70. doi: 10.1007/978-1-59745-019-5_11.
10
Isolation of mouse mammary epithelial subpopulations: a comparison of leading methods.小鼠乳腺上皮亚群分离：主流方法比较。
J Mammary Gland Biol Neoplasia. 2012 Jun;17(2):91-7. doi: 10.1007/s10911-012-9257-1. Epub 2012 May 30.
本文引用的文献
1
Spermidine in Regenerating Liver: Relation to Rapid Synthesis of Ribonucleic Acid.精胺在肝脏再生中的作用：与核糖核酸的快速合成有关。
Science. 1965 Jul 23;149(3682):428-9. doi: 10.1126/science.149.3682.428.
2
Putrescine and related compounds as growth factors for Hemophilus parainfluenzae 7991.腐胺及相关化合物作为副流感嗜血杆菌7991的生长因子
J Biol Chem. 1949 Nov;181(1):47-54.
3
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
4
5
Regulation of the activity of ornithine decarboxylase and S-adenosylmethionine decarboxylase in mammary gland and liver of lactating rats. Effects of starvation, prolactin and insulin deficiency.泌乳大鼠乳腺和肝脏中鸟氨酸脱羧酶及S-腺苷甲硫氨酸脱羧酶活性的调节。饥饿、催乳素和胰岛素缺乏的影响。
Biochem J. 1982 Mar 15;202(3):693-8. doi: 10.1042/bj2020693.
6
Induction of mammary gland carcinomas by the subcutaneous injection of 1-methyl-1-nitrosourea.通过皮下注射1-甲基-1-亚硝基脲诱导乳腺癌
Cancer Res. 1983 Apr;43(4):1628-9.
7
Polyamine metabolism and function.多胺代谢与功能。
Am J Physiol. 1982 Nov;243(5):C212-21. doi: 10.1152/ajpcell.1982.243.5.C212.
8
Susceptibility of the mammary gland to carcinogenesis. III. The cell of origin of rat mammary carcinoma.乳腺对致癌作用的易感性。III. 大鼠乳腺癌的起源细胞。
Am J Pathol. 1983 Oct;113(1):50-66.
9
Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats.饥饿和再喂养对泌乳大鼠乳腺中多胺浓度及鸟氨酸脱羧酶抗酶的影响。
Biochem J. 1983 Apr 15;212(1):149-53. doi: 10.1042/bj2120149.
10
Role of polyamines in the control of cell proliferation and differentiation.多胺在细胞增殖与分化调控中的作用。
Differentiation. 1981;19(1):1-20. doi: 10.1111/j.1432-0436.1981.tb01123.x.
Zotero 插件