Hirsch S, Gordon S
Immunobiology. 1982 Apr;161(3-4):298-307. doi: 10.1016/S0171-2985(82)80086-7.
Monoclonal antibodies have been used to study receptors on the plasma membrane of macrophages, as well as the processes of membrane synthesis, internalization, recycling and macrophage differentiation and activation. Various immunisation and screening procedures have been employed. Most reagents produced so far are not restricted to macrophages in their binding specificity. Monoclonal antibodies have been of great use in characterising murine Fc receptors and in studying the composition of the membrane of pinocytic vesicles. Antibody F4/80 has been used to examine the behaviour of the 160K membrane protein it defines during macrophage differentiation and activation. This antigen is absent on macrophage precursors, is expressed in relatively large amounts on mature macrophages, but expression is diminished on activated populations. No macrophage subset heterogeneity has been defined by ag F4/80 or 2 other monoclonal antibody-defined macrophage antigens, as all bone marrow-derived macrophage clones express these antigens. Anti-macrophage antibodies have already proved useful as diagnostic markers and in cell separation. Reagents such as these will help to sort out relationships between macrophages and other presumptive mononuclear phagocytes.
单克隆抗体已被用于研究巨噬细胞质膜上的受体，以及膜合成、内化、再循环和巨噬细胞分化与激活的过程。已采用了各种免疫和筛选程序。到目前为止产生的大多数试剂在结合特异性上并不局限于巨噬细胞。单克隆抗体在表征小鼠Fc受体和研究胞饮小泡膜的组成方面非常有用。抗体F4/80已被用于检测它所定义的160K膜蛋白在巨噬细胞分化和激活过程中的行为。这种抗原在巨噬细胞前体上不存在，在成熟巨噬细胞上大量表达，但在激活群体上表达减少。ag F4/80或其他两种单克隆抗体定义的巨噬细胞抗原未定义巨噬细胞亚群的异质性，因为所有骨髓来源的巨噬细胞克隆都表达这些抗原。抗巨噬细胞抗体已被证明可作为诊断标志物和用于细胞分离。这类试剂将有助于理清巨噬细胞与其他假定的单核吞噬细胞之间的关系。
