Matsuki Y, Ito T, Fukuhara K, Nakamura T, Kimura M, Ono H
J Chromatogr. 1982 Apr 30;239:585-94. doi: 10.1016/s0021-9673(00)82016-6.
A gas chromatographic-mass spectrometric method for the simultaneous determination of captopril (SQ 14,255) and its disulphide (SQ 14,551) in biological fluids by means of selected ion monitoring is described. In order to prevent oxidative degradation, captopril was treated with N-ethylmaleimide (NEM). The captopril-NEM adduct and the disulphide were converted into the hexafluoroisopropyl esters, which were separated on a 10% Dexsil 300 GC column and determined by employing the captopril-N-butylmaleimide adducts as an internal standard. The blood and urine levels of captopril and its disulphide in dogs to which captopril had been administered orally were measured by the proposed method. The urinary excretion of these two substances in rats was also determined in a similar manner.
本文描述了一种气相色谱 - 质谱联用方法，通过选择离子监测同时测定生物体液中的卡托普利（SQ 14,255）及其二硫化物（SQ 14,551）。为防止氧化降解，卡托普利用N - 乙基马来酰亚胺（NEM）处理。卡托普利 - NEM加合物和二硫化物被转化为六氟异丙酯，在10% Dexsil 300气相色谱柱上分离，并以卡托普利 - N - 丁基马来酰亚胺加合物作为内标进行测定。用所提出的方法测量了口服卡托普利的犬体内卡托普利及其二硫化物的血药浓度和尿药浓度。还以类似方式测定了大鼠体内这两种物质的尿排泄量。
