Meier D, Schachner M
J Neurosci Res. 1982;7(2):135-45. doi: 10.1002/jnr.490070205.
Oligodendrocytes isolated by immunoaffinity on magnetic beads using a monoclonal antibody to oligodendrocyte cell surfaces were obtained with a purity of 91 +/- 4% [see companion paper, Meier et al, 1982]. Contaminating cells consisted mostly of tetanus toxin-positive neurons with small cell bodies. Very few glial fibrillary acidic protein-positive astrocytes and fibronectin-positive fibroblasts or fibroblast-like cells could be detected by indirect immunofluorescence. Macrophages, as identified by their characteristic morphology, were rarely detectable in the cultures. With increasing time in culture, the degree of homogeneity of the cultured oligodendrocytes increased slightly, while the cell density of oligodendrocytes decreased drastically within the first week of culture. Cultures of immunoselected oligodendrocytes with a lower degree of purity showed a better survival of oligodendrocytes, but a considerable reduction in purity after three weeks in culture, probably due to overgrowth of nonoligodendroglial elements.
使用针对少突胶质细胞表面的单克隆抗体通过磁珠免疫亲和分离得到的少突胶质细胞，纯度为91±4%[见配套论文，Meier等人，1982年]。污染细胞主要由细胞体较小的破伤风毒素阳性神经元组成。通过间接免疫荧光法几乎检测不到胶质纤维酸性蛋白阳性星形胶质细胞以及纤连蛋白阳性成纤维细胞或成纤维细胞样细胞。根据其特征形态鉴定的巨噬细胞在培养物中很少能检测到。随着培养时间的增加，培养的少突胶质细胞的同质性程度略有增加，而少突胶质细胞的细胞密度在培养的第一周内急剧下降。纯度较低的免疫选择少突胶质细胞培养物显示少突胶质细胞的存活率更高，但培养三周后纯度大幅降低，这可能是由于非少突胶质细胞成分过度生长所致。
