Meier D H, Lagenaur C, Schachner M
J Neurosci Res. 1982;7(2):119-34. doi: 10.1002/jnr.490070204.
Oligodendrocytes from early postnatal mouse cerebellum were isolated using polyacrylamide-coated magnetic beads carrying monoclonal antibody to 04 cell surface antigen. Oligodendrocytes were enriched to a purity of 91 +/- 4% starting from a mixed cell population containing approximately 1.5% antigen-positive oligodendrocytes. Viability of 04 antigen-positive oligodendrocytes was approximately 90% as judged by exclusion of trypan blue. Oligodendrocytes were recovered after detachment from the beads with a yield of 19 +/- 6% and after collection by centrifugation onto glass coverslips with yields of approximately 6% of all 04 antigen-positive cells. The final cell yield of oligodendrocytes is approximately 8 x 10(5) cells/gram fresh cerebellar tissue.
使用携带抗04细胞表面抗原单克隆抗体的聚丙烯酰胺包被磁珠，从小鼠出生后早期的小脑分离少突胶质细胞。少突胶质细胞从含有约1.5%抗原阳性少突胶质细胞的混合细胞群体开始富集，纯度达到91±4%。通过台盼蓝排斥法判断，04抗原阳性少突胶质细胞的活力约为90%。少突胶质细胞在用磁珠分离后回收率为19±6%，通过离心收集到玻璃盖玻片上后，回收率约为所有04抗原阳性细胞的6%。少突胶质细胞的最终细胞产量约为8×10⁵个细胞/克新鲜小脑组织。
