Sandoz D, Gounon P, Karsenti E, Sauron M E
Proc Natl Acad Sci U S A. 1982 May;79(10):3198-202. doi: 10.1073/pnas.79.10.3198.
Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes are poorly stained. Actin antibodies clearly stain the axonemes, but only on the proximal-half portion, whereas myosin antibodies stain a small area of the axonemes just above the ciliary neck region.
通过使用纯化抗体，采用免疫细胞化学方法，在光学显微镜和电子显微镜下，已将微管蛋白、肌动蛋白和肌球蛋白定位到鹌鹑输卵管分离出的去膜纤毛细胞中。抗微管蛋白可使外周双联体和中央微管染色，而基体染色较差。肌动蛋白抗体能清晰地使轴丝染色，但仅在近端一半部分，而肌球蛋白抗体则使轴丝在纤毛颈部区域上方的一小片区域染色。
