Boisvieux-Ulrich E, Lainé M C, Sandoz D
Université Pierre et Marie Curie, Paris, France.
Cell Tissue Res. 1990 Mar;259(3):443-54. doi: 10.1007/BF01740770.
The effects of cytochalasin D (CD) were studied by scanning (SEM) and transmission (TEM) electron-microscopic examination at different stages of ciliary differentiation in epithelial cells of quail oviduct. Immature quails were prestimulated by estradiol benzoate injections to induce ciliogenesis in the undifferentiated oviduct. After 24 h of CD culture, SEM study revealed inhibition of ciliogenesis and dilation of the apex of non-ciliated cells. TEM study showed that 2 h of CD treatment produced dilation of lateral intercellular spaces, after 6 h of treatment, this resulted in intracellular macrovacuolation. Vacuoles were surrounded by aggregates of dense felt-like material. CD also induced the disappearance of microvilli, and rounding of the apical surface of undifferentiated cells and those blocked in ciliogenesis. Centriologenesis was not inhibited by CD; basal bodies assembled in generative complexes in the supranuclear region after 24 h of treatment. However, the migration of mature basal bodies towards the apical surface was impaired. Instead, they anchored onto the membrane of intracellular vacuoles; growth of cilia was induced in the vacuole lumen. Cilium elongation was disturbed, giving abnormally short cilia with a dilated tip; microtubules failed to organize correctly.
通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）检查，研究了细胞松弛素D（CD）对鹌鹑输卵管上皮细胞纤毛分化不同阶段的影响。未成熟鹌鹑通过注射苯甲酸雌二醇进行预刺激，以诱导未分化输卵管中的纤毛发生。CD培养24小时后，SEM研究显示纤毛发生受到抑制，未纤毛化细胞的顶端扩张。TEM研究表明，CD处理2小时后，细胞间侧间隙扩张，处理6小时后，导致细胞内出现大空泡。空泡被致密毡状物质聚集物包围。CD还诱导微绒毛消失，未分化细胞以及纤毛发生受阻的细胞顶端表面变圆。CD未抑制中心粒发生；处理24小时后，基体在核上区的生殖复合体中组装。然而，成熟基体向顶端表面的迁移受损。相反，它们锚定在细胞内空泡的膜上；在空泡腔内诱导纤毛生长。纤毛伸长受到干扰，产生顶端扩张的异常短纤毛；微管未能正确排列。
