Mikhaĭlov V I, Belen'kiĭ D M
Vopr Med Khim. 1982 May-Jun;28(3):48-50.
Treatment with sodium metaperiodate caused oxidation of carbohydrate residues in acid alpha-glucosidase (gamma-amylase). Efficiency of the oxidation was monitored by a decrease in specific binding of alpha-glucosidase with concanavaline A-Sepharose. The degree of oxidation reached 96% in a medium containing 0,011 M NaIO4, pH 5.5, within 120 min. The residual activity of oxidized alpha-glucosidase was about 77%. The rate of oxidation and residual enzymatic activity were shown to depend also on concentration of sodium metaperiodate and duration of the reaction.
用偏高碘酸钠处理导致酸性α-葡萄糖苷酶（γ-淀粉酶）中的碳水化合物残基氧化。通过α-葡萄糖苷酶与伴刀豆球蛋白A-琼脂糖的特异性结合减少来监测氧化效率。在含有0.011 M高碘酸钠、pH 5.5的培养基中，120分钟内氧化程度达到96%。氧化后的α-葡萄糖苷酶的残余活性约为77%。结果表明，氧化速率和残余酶活性还取决于偏高碘酸钠的浓度和反应持续时间。
