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长爪沙鼠幽门窦内分泌细胞的超微结构与细胞化学研究

An ultrastructural and cytochemical study of enteroendocrine cells of the pyloric antrum in the gerbil, Meriones unguiculatus.

作者信息

Kapur S P

出版信息

Acta Anat (Basel). 1982;112(3):220-32. doi: 10.1159/000145514.

DOI:10.1159/000145514
PMID:7048844
Abstract

Enteroendocrine cells of the gastric antrum have been examined in the gerbil, Meriones unguiculatus. Gastrin-secreting G cells were identified by immunocytochemical methods and by transmission electron microscopy. They were present in the lower and middle third of the pyloric antral glands. G cells were polyhedral in shape and often extended to the gland lumen where they presented a microvillous and pinocytotic surface. These cells were characterized by numerous electron-dense and immunoreactive secretory granules. Enterochromaffin cells were identified by a serotonin-specific formaldehyde-induced fluorescence method, and by electron microscopy which revealed pleomorphic secretory granules of varying electron densities. These cells also had luminal projections and had a shape and distribution essentially similar to those of the G cells. A third endocrine cell type was identified as Dl cell in the pyloric glands. This cell type was recognized by the presence of numerous secretory granules having an electron-dense core and a clear peripheral space, as also by the presence of microfilament bundles, especially in the perinuclear region. The functional correlates of these morphological observations are discussed with reference to their secretory product and the manner of its release, especially by the G cells.

摘要

在长爪沙鼠(Meriones unguiculatus)中对胃窦的肠内分泌细胞进行了研究。通过免疫细胞化学方法和透射电子显微镜鉴定出分泌胃泌素的G细胞。它们存在于幽门窦腺的下三分之一和中三分之一处。G细胞呈多面体形状,常常延伸至腺腔,在那里它们呈现出微绒毛状和胞饮表面。这些细胞的特征是有许多电子致密且具有免疫反应性的分泌颗粒。通过血清素特异性甲醛诱导荧光法以及电子显微镜鉴定出肠嗜铬细胞,电子显微镜显示出具有不同电子密度的多形性分泌颗粒。这些细胞也有腔面突起,其形状和分布与G细胞基本相似。在幽门腺中鉴定出第三种内分泌细胞类型为D1细胞。这种细胞类型通过存在许多具有电子致密核心和清晰周边空间的分泌颗粒以及通过存在微丝束(特别是在核周区域)来识别。结合这些细胞的分泌产物及其释放方式,特别是G细胞的释放方式,对这些形态学观察结果的功能相关性进行了讨论。

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