Varndell I M, Harris A, Tapia F J, Yanaihara N, De Mey J, Bloom S R, Polak J M
Experientia. 1983 Jul 15;39(7):713-7. doi: 10.1007/BF01990288.
Gastrin (G)-producing cells from the mammalian gastric antrum have been investigated using computer-assisted morphometry and a novel double colloidal gold-labeled-immunoglobulin electron immunocytochemical procedure. Correlation analysis of human antral G-cells indicates (p less than 0.001) that a single population of granules exists with small (160 nm) electron-dense and large (240 nm) electron-lucent forms representing the extremes. Non-crossreacting region-specific antisera have been used to visualize G-17 and G-34 (progastrin) to the small electron-dense granules and G-17 to the other intermediate forms. From the results we propose a topographic segregation of immunoreactive gastrins within 2 apparently distinct granule subclasses and suggest that this may represent the pathway of granule maturation.
利用计算机辅助形态测定法和一种新型双胶体金标记免疫球蛋白电子免疫细胞化学方法，对来自哺乳动物胃窦的胃泌素（G）产生细胞进行了研究。对人胃窦G细胞的相关性分析表明（p<0.001），存在单一群体的颗粒，有小的（160nm）电子致密型和大的（240nm）电子透亮型，代表了两种极端情况。已使用非交叉反应的区域特异性抗血清来观察小电子致密颗粒中的G-17和G-34（前胃泌素）以及其他中间形式中的G-17。根据结果，我们提出免疫反应性胃泌素在2种明显不同的颗粒亚类中的地形学分离，并表明这可能代表颗粒成熟的途径。
