Phillippe M, Niloff J M
Am J Obstet Gynecol. 1982 Aug 1;143(7):782-7. doi: 10.1016/0002-9378(82)90010-2.
Previous investigation has demonstrated biologically significant concentrations of catecholamines in amniotic fluid, which increase with gestation. The half life, metabolic clearance rate, and metabolic fate of these hormones in the amniotic compartment are yet to be established. This study was undertaken to demonstrate the ability of human chorioamnion to metabolize dopamine in vitro. Incubation experiments demonstrated that 3H-dopamine is rapidly metabolized to dihydroxyphenylacetic acid, 3-methoxy, 4-hydroxyphenylacetic acid, and 3-methoxy, 4-hydroxyphenylethanol-all products of monoamine oxidase. No significant 3-methoxytyramine, a catechol-o-methyltransferase product, was observed. Incubation experiments with pargyline, a monoamine oxidase inhibitor, resulted in significant reduction in 3H-dopamine metabolism. Catecholamines and their interaction with prostaglandin synthesis have been theorized to be a fetal signal for the initiation of parturition. The ability of chorioamnion to metabolize catecholamine could, therefore, provide another control mechanism by which fetal catecholamines are modulated.
先前的研究已证实在羊水中存在具有生物学意义的儿茶酚胺浓度，且该浓度会随着孕周增加而升高。这些激素在羊膜腔中的半衰期、代谢清除率及代谢转归尚未明确。本研究旨在证实人绒毛膜羊膜在体外代谢多巴胺的能力。孵育实验表明，³H-多巴胺可迅速代谢为二羟苯乙酸、3-甲氧基-4-羟基苯乙酸以及3-甲氧基-4-羟基苯乙醇——这些均为单胺氧化酶的产物。未观察到显著量的儿茶酚-O-甲基转移酶产物3-甲氧基酪胺。使用单胺氧化酶抑制剂帕吉林进行的孵育实验导致³H-多巴胺代谢显著减少。儿茶酚胺及其与前列腺素合成的相互作用被认为是启动分娩的胎儿信号。因此，绒毛膜羊膜代谢儿茶酚胺的能力可能提供了另一种调节胎儿儿茶酚胺的控制机制。
