Thacker W L, Lewis V J, Shaddock J H, Winkler W G
Am J Vet Res. 1982 Aug;43(8):1500-2.
The prevention of hamster-associated outbreaks of lymphocytic choriomeningitis virus infection in human beings requires rapid and reliable testing of large numbers of hamsters for the infection. To select the most effective test, the antibody response of infected hamsters was determined by the indirect fluorescent antibody and complement-fixation techniques. The indirect fluorescent antibody technique required less than 2 hours to complete, was the first to become positive after infection, and remained positive for at least several months. Infection in hamsters was also readily detected by the inoculation of mice with infected hamster tissues; virus could be isolated from several organs as early as postinoculation day (PID) 3, and all organs tested contained high concentrations of virus by PID 5. After PID 40, virus was detectable only in the kidney; this organ remained positive for over 3 months.
预防人类中由仓鼠引发的淋巴细胞性脉络丛脑膜炎病毒感染的爆发，需要对大量仓鼠进行快速可靠的感染检测。为了选择最有效的检测方法，通过间接荧光抗体和补体结合技术测定了受感染仓鼠的抗体反应。间接荧光抗体技术完成检测所需时间不到2小时，是感染后最早呈阳性的方法，并且至少几个月内一直呈阳性。通过将受感染仓鼠的组织接种到小鼠体内，也能很容易地检测出仓鼠的感染情况；早在接种后第3天就能从多个器官中分离出病毒，到接种后第5天，所有检测的器官都含有高浓度的病毒。接种后40天之后，仅在肾脏中可检测到病毒；该器官在3个多月内一直呈阳性。
