以重组核蛋白为抗原，采用酶联免疫吸附测定法检测小鼠血清中淋巴细胞性脉络丛脑膜炎病毒抗体。
Enzyme-linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen.
作者信息
Homberger F R, Romano T P, Seiler P, Hansen G M, Smith A L
机构信息
Institute of Laboratory Animal Science, University of Zurich, Switzerland.
出版信息
Lab Anim Sci. 1995 Oct;45(5):493-6.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibody to lymphocytic choriomeningitis virus (LCMV) in mouse sera. This assay is based on recombinant LCMV nucleoprotein generated in a baculovirus system. Sera from experimentally and naturally infected as well as noninfected mice were tested, and the results were compared with those obtained from an established immunofluorescence assay (IFA) that uses infected cells as antigen. An excellent correlation was found; the ELISA specificity and sensitivity were calculated to be 100 and 95% respectively. Unlike the IFA, this ELISA does not require the handling of infective virus. It eliminates the need to work with a zoonotic agent in the laboratory while allowing effective screening of laboratory mouse populations for LCMV antibody.
开发了一种酶联免疫吸附测定（ELISA）方法，用于检测小鼠血清中淋巴细胞性脉络丛脑膜炎病毒（LCMV）抗体。该测定基于杆状病毒系统中产生的重组LCMV核蛋白。对实验感染、自然感染以及未感染小鼠的血清进行了检测，并将结果与使用感染细胞作为抗原的既定免疫荧光测定（IFA）所获得的结果进行了比较。发现两者具有极好的相关性；ELISA的特异性和敏感性经计算分别为100%和95%。与IFA不同，该ELISA不需要处理感染性病毒。它消除了在实验室中处理人畜共患病原体的需求，同时允许对实验室小鼠群体进行有效的LCMV抗体筛查。
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