Anhut H, Bernauer W, Peskar B A
Prostaglandins. 1978 May;15(5):889-900. doi: 10.1016/0090-6980(78)90156-9.
Isolated perfused sensitized guinea pig hearts release relatively large amounts of radioimmunologically measurable thromboxane B2 (TXB2) as well as smaller amounts of prostaglandin (PGs) after antigenic challenge. Using thin layer chromatography the major PG released was shown to cochromatograph with PGD2, while smaller amounts of immunoreactive PGF2alpha were found. The TX-synthetase inhibitor imidazole (100 microgram/ml) significantly decreased TXB2 release and simultaneously increased PG release during cardiac anaphylaxis. On the other hand, the beta-sympathomimetic drug isoproterenol decreased both TXB2 and PG release from the anaphylactic hearts. While isoproterenol significantly diminished anaphylactic coronary flow reduction, imidazole was without effect in this respect. PGD2 (0.5 microgram/min and 5.0 microgram/min) infused intraaortally into non-sensitized guinea pig hearts reduced coronary flow dose-dependently. These results are compatible with the view that release of TX and PGs might contribute to coronary flow reduction in cardiac anaphylaxis.
抗原攻击后，分离灌注的致敏豚鼠心脏会释放相对大量的可通过放射免疫法检测的血栓素B2（TXB2）以及较少量的前列腺素（PGs）。通过薄层层析法显示，释放的主要PG与PGD2共层析，同时发现较少量的免疫反应性PGF2α。TX合成酶抑制剂咪唑（100微克/毫升）在心脏过敏反应期间显著降低TXB2释放，同时增加PG释放。另一方面，β-拟交感神经药物异丙肾上腺素减少了过敏心脏中TXB2和PG的释放。虽然异丙肾上腺素显著减轻了过敏反应导致的冠状动脉血流减少，但咪唑在这方面没有作用。将PGD2（0.5微克/分钟和5.0微克/分钟）经主动脉内注入未致敏的豚鼠心脏会使冠状动脉血流呈剂量依赖性减少。这些结果与以下观点一致，即TX和PGs的释放可能导致心脏过敏反应中冠状动脉血流减少。
