互相关激光散射
Cross-correlation laser scattering.
作者信息
Kam Z, Rigler R
出版信息
Biophys J. 1982 Jul;39(1):7-13. doi: 10.1016/S0006-3495(82)84484-6.
Abstract
Cross-correlation between two detectors was applied to analyze laser light-scattering fluctuations. Laser scattering from random concentration fluctuations is spatially coherent over small angular areas that are inversely proportional in size to the dimension of the scattering volume. By cross-correlating scattering intensity fluctuations in different angles, the correlation due to relaxation of concentration fluctuations is practically eliminated, and correlations reflecting changes in the scattering from the individual particles can be enhanced. Rotational diffusion of assymetric particles, conformational relaxation of random coils, and association-dissociation dynamics are determined here using the above approach.
摘要
应用两个探测器之间的互相关来分析激光散射波动。来自随机浓度波动的激光散射在小角度区域内是空间相干的，这些区域的大小与散射体积的尺寸成反比。通过对不同角度的散射强度波动进行互相关，可以有效消除由于浓度波动弛豫引起的相关性，并增强反映单个粒子散射变化的相关性。本文使用上述方法确定不对称粒子的旋转扩散、无规卷曲的构象弛豫以及缔合-解离动力学。
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