Interactions of lipopolysaccharides, glycolipid, lipid A with acid soluble basement membrane collagen during thermal aggregation.

Lipopolysaccharides derived from a number of Escherichia coli and Salmonella species were incorporated into thermal aggregates acid soluble basement membrane collagens. Although most of these endotoxins were noted to inhibit basement membrane collagen aggregation, some lipopolysaccharides possessed little or no inhibitory properties. However, the lipid A derived from all of the latter lipopolysaccharides and the glycolipid from Salmonella minnesota R595 were noted to be significant inhibitors of acid soluble basement membrane collagen aggregation. These data suggest that the nonpolar region of the endotoxin molecule is responsible for the inhibition of acid soluble basement membrane collagen assemblies and that these intermolecular interactions probably occur with the hydrophobic globular domain of acid soluble basement membrane collagens.