Bours J, Vornhagen R, Herlt M, Rink H
Curr Eye Res. 1981;1(11):651-8. doi: 10.3109/02713688109001869.
Calf gamma-crystallins were separated by preparative flat-bed isoelectric focusing and characterized according to their immunological properties, molecular weights and isoelectric points. Immunologically 4 different groups of gamma-crystallins (gamma 1a-1d-crystallin) could be detected. The main immunological component, the gamma 1b-crystallin corresponds to two polypeptides with molecular weights of 20,500 and 22,000. The two minor components, the gamma 1c- and gamma 1d-crystallin, are immunologically partial identical with the main component gamma 1b-crystallin. One of these minor components, the gamma 1d-crystallin, has a MW of 29,000. The 4th antigen (gamma 1a-crystallin), which has a MW of 21,000, seems to have only remote relationship with the major gamma-crystallin component.
通过制备型平板等电聚焦法分离小牛γ-晶体蛋白，并根据其免疫特性、分子量和等电点进行表征。在免疫学上可检测到4种不同的γ-晶体蛋白组（γ1a - 1d - 晶体蛋白）。主要的免疫成分γ1b - 晶体蛋白对应于分子量为20,500和22,000的两种多肽。两种次要成分γ1c - 晶体蛋白和γ1d - 晶体蛋白在免疫学上与主要成分γ1b - 晶体蛋白部分相同。这些次要成分之一γ1d - 晶体蛋白的分子量为29,000。第4种抗原（γ1a - 晶体蛋白）的分子量为21,000，似乎与主要的γ-晶体蛋白成分只有较远的关系。
