Amaldi F, Beccari E, Bozzoni I, Luo Z X, Pierandrei-Amaldi P
Gene. 1982 Mar;17(3):311-6. doi: 10.1016/0378-1119(82)90147-0.
We have previously constructed and selected six recombinant plasmids containing cDNA sequences specific for different ribosomal proteins of Xenopus laevis (Bozzoni et al., 1981). DNA cloned in these plasmids have been isolated and sequenced. Amino acid sequences of the corresponding portions of the proteins have been derived from DNA sequences; they are arginine- and lysine-rich as expected for ribosomal proteins. One of the cDNA sequences has an open reading frame also on the strand complementary to the one coding for the ribosomal protein; this fragment has inverted repeats twenty nucleotides lone at the two ends. The codon usage for the six sequences appears to be non-random with some differences among the ribosomal proteins analysed.
我们之前构建并筛选出了六个重组质粒，它们包含非洲爪蟾不同核糖体蛋白的特异性cDNA序列（博佐尼等人，1981年）。克隆于这些质粒中的DNA已被分离并测序。蛋白质相应部分的氨基酸序列已从DNA序列推导得出；正如核糖体蛋白所预期的那样，它们富含精氨酸和赖氨酸。其中一个cDNA序列在与编码核糖体蛋白的链互补的链上也有一个开放阅读框；该片段在两端有20个核苷酸长的反向重复序列。这六个序列的密码子使用情况似乎并非随机，在所分析的核糖体蛋白之间存在一些差异。
