Taylor M A, Arif S A, Pearce S R, Davies H V, Kumar A, George L A
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom.
Plant Physiol. 1992 Nov;100(3):1171-6. doi: 10.1104/pp.100.3.1171.
cDNA clones of two genes, TUBS19 and TUBL7, which show a 15- to 20-fold increase in transcript level in the stolon tip during the early stages of tuberization, have been isolated by differential screening. These genes are also expressed in leaves, stems, and roots, and the expression pattern in these organs changes on tuberization. Southern analysis shows that there are similar sequences in the genome of nontuberizing wild-type potato species Solanum brevidens and in Lycopersicon esculentum (tomato). Sequence analysis reveals a high degree of similarity between the TUBS19 cDNA and the eukaryotic S19 ribosomal protein gene. TUBL7 cDNA shows similarity to another eukaryotic ribosomal protein gene, L7.
通过差异筛选分离出了两个基因TUBS19和TUBL7的cDNA克隆，这两个基因在块茎形成早期的匍匐茎顶端转录水平增加了15至20倍。这些基因也在叶、茎和根中表达，并且在块茎形成时这些器官中的表达模式会发生变化。Southern分析表明，在不形成块茎的野生型马铃薯物种短柄茄（Solanum brevidens）的基因组以及番茄（Lycopersicon esculentum）中存在相似序列。序列分析揭示了TUBS19 cDNA与真核生物S19核糖体蛋白基因之间高度相似。TUBL7 cDNA与另一个真核生物核糖体蛋白基因L7相似。
