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可扩增质粒pColA-CA31的物理图谱以及大肠杆菌素A结构基因的位置。

Physical map of pColA-CA31, an amplifiable plasmid, and location of colicin A structural gene.

作者信息

Morlon J, Cavard D, Lazdunski C

出版信息

Gene. 1982 Mar;17(3):317-21. doi: 10.1016/0378-1119(82)90148-2.

DOI:10.1016/0378-1119(82)90148-2
PMID:7049840
Abstract

Evidence showing that the plasmic ColA, derived from strain CA31[pColA] can be amplified in the presence of chloramphenicol is presented. This plasmid has been purified and its Mr-value has been found to be 4.6 X 10(6) or 7 kb. Twelve cleavage sites have been mapped in pColA by using single and double restriction endonuclease digestions. These sites were ordered in relation to the single HindIII site. The other restriction endonucleases used were, respectively, SmaI, AvaI, PstI and HincII. Establishment of the map was helped by hybridization of pColA endonuclease digest products with 32P-labeled colicin A-mRNA. The structural gene for colicin A was contained in a 2.17-kb HincII fragment.

摘要

有证据表明，源自菌株CA31[pColA]的胞质ColA在氯霉素存在的情况下可以扩增。该质粒已被纯化，其分子量值为4.6×10(6)或7 kb。通过单酶切和双酶切，已在pColA中定位了12个切割位点。这些位点相对于单一的HindIII位点进行了排序。使用的其他限制性内切酶分别是SmaI、AvaI、PstI和HincII。pColA内切酶消化产物与32P标记的大肠杆菌素A-mRNA杂交有助于构建图谱。大肠杆菌素A的结构基因包含在一个2.17 kb的HincII片段中。

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1
Physical map of pColA-CA31, an amplifiable plasmid, and location of colicin A structural gene.可扩增质粒pColA-CA31的物理图谱以及大肠杆菌素A结构基因的位置。
Gene. 1982 Mar;17(3):317-21. doi: 10.1016/0378-1119(82)90148-2.
2
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大肠杆菌素A结构基因caa的启动子、操纵基因及氨基末端区域的核苷酸序列
EMBO J. 1983;2(5):787-9. doi: 10.1002/j.1460-2075.1983.tb01501.x.
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Mol Gen Genet. 1983;192(3):500-5. doi: 10.1007/BF00392196.
5
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Mol Gen Genet. 1985;199(1):95-100. doi: 10.1007/BF00327516.
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