abi、col和imm基因在pHU011（一种大肠杆菌素Ib质粒衍生物）上的定位。

Location of the abi, col and imm genes on pHU011, a colicin Ib plasmid derivative.

作者信息

Gottlieb J H, Duckworth D H

出版信息

Gene. 1983 Dec;26(2-3):301-2. doi: 10.1016/0378-1119(83)90201-9.

Abstract

A cleavage site map of pHU011, a derivative of the colicin Ib plasmid containing the complete SalI-B fragment ligated to pBR322, has been determined. Sites of cleavage by PstI were determined using the Smith and Birnstiel [Nucl. Acids Res. 3 (1976) 2387-2398] method of mapping, whereas those for XbaI, XhoI, and HindIII were determined by double digestions or digestion of isolated fragments. In addition, the sites of the abi gene, which causes the abortive infection by T5 bacteriophage, and of the colicin (col) gene have been determined. The results indicate that these genes are not contiguous.

摘要

已确定pHU011的切割位点图谱，pHU011是含有与pBR322连接的完整SalI - B片段的大肠杆菌素Ib质粒的衍生物。使用史密斯和伯恩斯蒂尔[《核酸研究》3 (1976) 2387 - 2398]的图谱绘制方法确定了PstI的切割位点，而XbaI、XhoI和HindIII的切割位点则通过双酶切或分离片段的酶切来确定。此外，还确定了导致T5噬菌体流产感染的abi基因以及大肠杆菌素（col）基因的位点。结果表明这些基因不相邻。