Borcić B, Kovacić H, Sebek Z, Aleraj B, Tvrtković N
Folia Parasitol (Praha). 1982;29(2):177-82.
Small mammals trapped in seven sites along the river Sava in Croatia were examined for leptospiral infection by renoculture and serological methods. Of the 1749 animals caught 702 were examined by renoculture method and 626 were tested by microagglutination-lysis. The dominant animals species caught were Microtus arvalis (672 animals) and Apodemus agrarius (552 animals). Of the 115 strains of leptospires isolated the dominant serotypes were L. grippotyphosa (40) and L. pomona (35). Five strains of L. sejroe, 9 strains of L. australis, 1 strain of L. bataviae and 25 undetermined strains were isolated, the infection rate ranging from 3 to 32%. Serologic evidence of infection was demonstrated in 91 animals, the prevalence ranging from 0 to 43% of animals depending on the trapping site. Specific agglutinins most frequently encountered were those against L. pomona (50) and L. grippotyphosa (26), though agglutinins against L. jalna (5), L. bataviae (4), L. bratislava (3) etc., were also found. A high degree if association between L. grippotyphosa and the common vole as well as between L. pomona and the field mouse was found indicating that as far as these two types of leptospires are concerned the Sava valley may be considered as their vast natural focus. It was also concluded that wild small mammals in this region of Croatia may be a source of leptospiral infection for the local population as well as for outdoor-reared domestic animals (swine, bovine).
通过肾脏培养和血清学方法，对在克罗地亚萨瓦河沿岸七个地点捕获的小型哺乳动物进行钩端螺旋体感染检测。在捕获的1749只动物中，702只通过肾脏培养法检测，626只通过微量凝集溶解试验检测。捕获的主要动物种类是普通田鼠（672只）和黑线姬鼠（552只）。在分离出的115株钩端螺旋体中，主要血清型为波摩那群（40株）和黄疸出血群（35株）。分离出5株赛罗群、9株澳洲群、1株巴达维亚群和25株未确定血清型的菌株，感染率在3%至32%之间。91只动物有感染的血清学证据，患病率因捕获地点而异，在0%至43%之间。最常遇到的特异性凝集素是针对波摩那群（50株）和黄疸出血群（26株）的，不过也发现了针对爪哇群（5株）、巴达维亚群（4株）、布拉迪斯拉发群（3株）等的凝集素。发现黄疸出血群与普通田鼠以及波摩那群与田鼠之间有高度关联，这表明就这两种钩端螺旋体而言，萨瓦河谷可被视为它们广阔的自然疫源地。还得出结论，克罗地亚这一地区的野生小型哺乳动物可能是当地居民以及户外饲养的家畜（猪、牛）钩端螺旋体感染的来源。
