Stewart V
J Bacteriol. 1982 Sep;151(3):1320-5. doi: 10.1128/jb.151.3.1320-1325.1982.
I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.
我使用了一个chlC-lac操纵子融合体来研究影响大肠杆菌中硝酸盐还原酶表达的调控突变。一个NarL突变体显然缺乏硝酸盐特异性的正调控成分。此外,一个fnr(nirR)突变在任何条件下都阻止了酶的诱导。这些数据与硝酸盐还原酶调控的两步正调控模型一致。