narL基因产物可激活大肠杆菌中的硝酸还原酶操纵子，并抑制延胡索酸还原酶和三甲胺N-氧化物还原酶操纵子。
The narL gene product activates the nitrate reductase operon and represses the fumarate reductase and trimethylamine N-oxide reductase operons in Escherichia coli.
作者信息
Iuchi S, Lin E C
出版信息
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3901-5. doi: 10.1073/pnas.84.11.3901.
Abstract
Escherichia coli, which can utilize O2, nitrate, fumarate, or trimethylamine N-oxide (Me3NO) as terminal electron acceptor, preferentially utilizes the one with the highest redox potential. Thus O2 prevents induction of nitrate, fumarate, and Me3NO reductases, and nitrate curtails the induction of fumarate and Me3NO reductases. Under anaerobic conditions the narL gene product, in the presence of nitrate, is known to activate transcription of the narC operon, which encodes nitrate reductase. This study shows that the same product plays a role in the repression by nitrate of the operons (frd and tor) that encode fumarate and Me3NO reductases. In contrast, the anaerobic repression of ethanol dehydrogenase by nitrate does not require the narL product. Expression of narL does not require the fnr gene product, a pleiotropic activator that is required for full expression of narC, frd, and tor.
摘要
大肠杆菌能够利用氧气、硝酸盐、富马酸盐或三甲胺 N-氧化物（Me3NO）作为末端电子受体，它优先利用氧化还原电位最高的那种物质。因此，氧气会阻止硝酸盐、富马酸盐和 Me3NO 还原酶的诱导合成，而硝酸盐会抑制富马酸盐和 Me3NO 还原酶的诱导合成。在厌氧条件下，已知在有硝酸盐存在时，narL 基因产物会激活 narC 操纵子的转录，narC 操纵子编码硝酸盐还原酶。这项研究表明，同一产物在硝酸盐对编码富马酸盐和 Me3NO 还原酶的操纵子（frd 和 tor）的阻遏中发挥作用。相比之下，硝酸盐对乙醇脱氢酶的厌氧阻遏不需要 narL 产物。narL 的表达不需要 fnr 基因产物，fnr 基因产物是一种多效激活剂，narC、frd 和 tor 的充分表达都需要它。
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The narL gene product activates the nitrate reductase operon and represses the fumarate reductase and trimethylamine N-oxide reductase operons in Escherichia coli.narL基因产物可激活大肠杆菌中的硝酸还原酶操纵子，并抑制延胡索酸还原酶和三甲胺N-氧化物还原酶操纵子。
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3901-5. doi: 10.1073/pnas.84.11.3901.
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