Gerdes J, Stein H, Dierich M P, Conrad D H
J Immunol Methods. 1982 Jun 11;51(2):259-65. doi: 10.1016/0022-1759(82)90265-4.
Antisera directed at human C3 receptors were prepared by 2 different methods. In method I rabbits were immunized with EAC coated with C3 receptors of tonsil cells (AC3RS type I). In method II rabbits were immunized with insoluble aggregates of tonsil membrane constituents containing significant amounts of C3 receptors. After appropriate absorptions both types of AC3RS showed a selective reactivity with those cells known to express C3 receptors. With the EAC rosette inhibition test it is demonstrated that both types of AC3RS contain antibodies against the binding sites of C3b, C3bi and C3d receptors. The antibody titer was consistently higher in AC3RS of type II.
针对人C3受体的抗血清通过两种不同方法制备。方法I中，用包被有扁桃体细胞C3受体的EAC（I型AC3RS）免疫兔子。方法II中，用含有大量C3受体的扁桃体膜成分的不溶性聚集体免疫兔子。经过适当吸收后，两种类型的AC3RS均显示出与已知表达C3受体的细胞具有选择性反应性。通过EAC玫瑰花结抑制试验证明，两种类型的AC3RS均含有针对C3b、C3bi和C3d受体结合位点的抗体。II型AC3RS的抗体效价始终较高。
