Yamakawa M, Imai Y
Second Department of Pathology, Yamagata University School of Medicine, Japan.
Immunology. 1992 Jul;76(3):378-84.
A comparative immunohistochemical study of the distribution pattern of complement components and regulatory proteins within secondary lymphoid follicles was performed by the immunoperoxidase technique. Fifteen lymphoid tissues including appendices. Peyer's patches and tonsils were analysed. Sixty secondary lymphoid follicles with evident polarity, that is, the distinct coexistence of a light zone, dark zone and mantle zone in the same lymphoid follicle, were tested with single antibodies. The light zones were consistently immunostained in a dendritic meshwork pattern with all antibodies. The immunostaining patterns were classified into two major groups based on the immunoreactivity of the dark zone. One immunostaining pattern was characterized by no immunostaining of the dark zone to the majority of the antigens. The second group was characterized by a diffusely weak to moderate dendritic meshwork pattern of the dark zone to some of the immunostainings of C9 (monoclonal), S-protein, and DF-DRC1, and all immunostainings of CR1 (CD35), Ber-Mac-DRC (CD35), CR2 (CD21), and R4/23. All four complement regulatory proteins were localized by immunoelectron microscopy attached to the cell surface of the cells, including follicular dendritic cells, in the light zone. Our data indicate that there is an evident functional difference between the light zone and the dark zone, and that complete activation of the complement system occurs only in the light zone.
采用免疫过氧化物酶技术对次级淋巴滤泡内补体成分和调节蛋白的分布模式进行了比较免疫组化研究。分析了包括阑尾、派尔集合淋巴结和扁桃体在内的15个淋巴组织。用单克隆抗体检测了60个具有明显极性的次级淋巴滤泡，即在同一淋巴滤泡中明显共存的明区、暗区和套区。所有抗体对明区的免疫染色均呈树突状网状模式。根据暗区的免疫反应性，将免疫染色模式分为两大类。一种免疫染色模式的特征是暗区对大多数抗原无免疫染色。第二组的特征是暗区对C9（单克隆）、S蛋白和DF-DRC1的一些免疫染色呈弥漫性弱阳性至中度树突状网状模式，以及对CR1（CD35）、Ber-Mac-DRC（CD35）、CR2（CD21）和R4/23的所有免疫染色均呈此模式。通过免疫电子显微镜观察发现，所有四种补体调节蛋白均定位于明区内包括滤泡树突状细胞在内的细胞表面。我们的数据表明，明区和暗区之间存在明显的功能差异，补体系统的完全激活仅发生在明区。
