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来自小泰勒虫沉淀抗原的纯化与特性分析

Purification and characterization of precipitating antigens from Theileria parva.

作者信息

Allsopp B A, Wagner G G, Matthews K P, Kariavu C G

出版信息

J Gen Microbiol. 1977 Jun;100(2):319-28. doi: 10.1099/00221287-100-2-319.

DOI:10.1099/00221287-100-2-319
PMID:70504
Abstract

Precipitating antigens from Theileria parva have been partially purified. Two antigens from each of the schizont and piroplasm stages of the parasite were identified; the major antigens from the two stages shared the same specificity. The antigens showed considerable molecular heterogeneity, almost certainly a result of the preparative method, and they always contained large amounts of DNA. The piroplasm antigens were of parasite nuclear origin and the schizont antigens were probably of the same origin. The antigens were weakly antigenic, and the activity against them of humoral antibody from cattle immune to East Coast fever was low. These antigens do not appear to induce protection against East Coast fever.

摘要

来自牛泰勒虫的沉淀抗原已得到部分纯化。从该寄生虫的裂殖体和梨形虫阶段分别鉴定出两种抗原;这两个阶段的主要抗原具有相同的特异性。这些抗原表现出相当大的分子异质性,几乎可以肯定这是制备方法所致的结果,并且它们总是含有大量DNA。梨形虫抗原源自寄生虫细胞核,裂殖体抗原可能也来自同一来源。这些抗原的抗原性较弱,对东海岸热免疫的牛的体液抗体针对它们的活性较低。这些抗原似乎不能诱导针对东海岸热的保护作用。

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Purification and characterization of precipitating antigens from Theileria parva.来自小泰勒虫沉淀抗原的纯化与特性分析
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