紫外线在大肠杆菌中的突变特异性：DNA二级结构作用的迹象
Mutational specificity of UV light in Escherichia coli: indications for a role of DNA secondary structure.
作者信息
Todd P A, Glickman B W
出版信息
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4123-7. doi: 10.1073/pnas.79.13.4123.
Abstract
We used the lacI forward mutagenesis system to determine the mutational specificity of UV-induced mutation in a repair-proficient (Uvr+) and a repair-deficient (delta UvrB) strain of Escherichia coli. The spectra recovered at similar levels of mutagenesis were similar, the exception being a mutational hotspot at site A24 specific to the delta UvrB strain. Mutations induced at this hotspot, as well as those induced at other mutational hotspots that were found to be common to both the Uvr+ and Uvr- strains, involve G . C leads to A . T transitions. All of the hotspots are at sites of potential dipyrimidine photoproducts, such as thymine-cytosine and cytosine-cytosine dimers, or of pyrimidine-cytosine photoproduct Py-C lesions. Each of these hotspots occurs at a site in the potential hairpin loop of quasi-palindromic sequences. These observations suggest an important role for DNA structure in determining the fate of UV-induced premutational lesions.
摘要
我们使用乳糖抑制子正向诱变系统来确定紫外线诱导的突变在大肠杆菌的修复 proficient（Uvr+）和修复缺陷（ΔUvrB）菌株中的突变特异性。在相似诱变水平下恢复的光谱相似，唯一的例外是ΔUvrB菌株特有的A24位点的一个突变热点。在这个热点诱导的突变，以及在Uvr+和Uvr-菌株中都发现的其他突变热点诱导的突变，都涉及G.C到A.T的转换。所有热点都位于潜在的二嘧啶光产物位点，如胸腺嘧啶 - 胞嘧啶和胞嘧啶 - 胞嘧啶二聚体，或嘧啶 - 胞嘧啶光产物Py - C损伤位点。这些热点中的每一个都出现在准回文序列潜在发夹环的一个位点上。这些观察结果表明DNA结构在决定紫外线诱导的前突变损伤的命运中起重要作用。
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本文引用的文献
1
Cruciform structures in supercoiled DNA.超螺旋DNA中的十字形结构。
Nature. 1981 Feb 5;289(5797):466-70. doi: 10.1038/289466a0.
2
Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.紫外线诱导的人类DNA特定序列损伤的分布：嘧啶核苷 - 胞苷序列处碱敏感损伤的检测
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3388-92. doi: 10.1073/pnas.78.6.3388.
3
The inverted repeat as a recognizable structural feature in supercoiled DNA molecules.反向重复序列作为超螺旋DNA分子中一种可识别的结构特征。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6468-72. doi: 10.1073/pnas.77.11.6468.
4
Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus.藤黄微球菌的嘧啶二聚体DNA糖基化酶对特定DNA序列中嘧啶二聚体的切割作用
Nature. 1980 Jun 26;285(5767):634-41. doi: 10.1038/285634a0.
5
A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4.利用噬菌体T4的无义突变体测定细菌中的诱变特异性。
J Mol Biol. 1967 Jun 28;26(3):437-47. doi: 10.1016/0022-2836(67)90314-2.
6
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Virology. 1971 Feb;43(2):495-503. doi: 10.1016/0042-6822(71)90321-7.
7
Radiation-induced mutations and their repair.辐射诱导的突变及其修复。
Science. 1966 Jun 3;152(3727):1345-53. doi: 10.1126/science.152.3727.1345.
8
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9
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Mutat Res. 1970 Apr;9(4):349-58. doi: 10.1016/0027-5107(70)90017-5.
10
Different modes of loss of photoreversibility of mutation and lethal damage in ultraviolet-light resistant and sensitive bacteria.抗紫外线和紫外线敏感细菌中突变和致死损伤的光可逆性丧失的不同模式。
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