Fix D, Bockrath R
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4446-9. doi: 10.1073/pnas.80.14.4446.
We have tested the two-event model for UV mutagenesis producing class 2 suppressor mutations at glutamine tRNA genes in Escherichia coli. In the model used, the induction/indexing lesion is any type of pyrimidine dimer and the premutational photoproduct at the target site is a cytosine-containing dimer. Specific mutation-frequency responses were analyzed under conditions in which the ratio of thymine-thymine dimers to cytosine-containing dimers was modified by using 313-nm light and 0.0%, 0.1%, or 0.2% acetophenone. Changes observed in the production of class 2 suppressor mutations were consistent with the model and suggested that the G X C leads to A X T transitions responsible for class 2 suppressor mutations are targeted by cytosine-containing pyrimidine dimers at the mutational sites.
我们已经测试了双事件模型，该模型用于研究紫外线诱变在大肠杆菌谷氨酰胺tRNA基因中产生2类抑制突变的情况。在所使用的模型中，诱导/索引损伤是任何类型的嘧啶二聚体，而靶位点的预突变光产物是含胞嘧啶的二聚体。通过使用313纳米光以及0.0%、0.1%或0.2%的苯乙酮来改变胸腺嘧啶-胸腺嘧啶二聚体与含胞嘧啶二聚体的比例，在此条件下分析了特定的突变频率响应。在2类抑制突变产生过程中观察到的变化与该模型一致，这表明导致2类抑制突变的G X C到A X T的转变是由突变位点处含胞嘧啶的嘧啶二聚体作为靶点的。
