含胞嘧啶嘧啶二聚体的靶向突变：用苯乙酮和313纳米光对大肠杆菌B/r的研究
Targeted mutation at cytosine-containing pyrimidine dimers: studies of Escherichia coli B/r with acetophenone and 313-nm light.
作者信息
Fix D, Bockrath R
出版信息
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4446-9. doi: 10.1073/pnas.80.14.4446.
Abstract
We have tested the two-event model for UV mutagenesis producing class 2 suppressor mutations at glutamine tRNA genes in Escherichia coli. In the model used, the induction/indexing lesion is any type of pyrimidine dimer and the premutational photoproduct at the target site is a cytosine-containing dimer. Specific mutation-frequency responses were analyzed under conditions in which the ratio of thymine-thymine dimers to cytosine-containing dimers was modified by using 313-nm light and 0.0%, 0.1%, or 0.2% acetophenone. Changes observed in the production of class 2 suppressor mutations were consistent with the model and suggested that the G X C leads to A X T transitions responsible for class 2 suppressor mutations are targeted by cytosine-containing pyrimidine dimers at the mutational sites.
摘要
我们已经测试了双事件模型，该模型用于研究紫外线诱变在大肠杆菌谷氨酰胺tRNA基因中产生2类抑制突变的情况。在所使用的模型中，诱导/索引损伤是任何类型的嘧啶二聚体，而靶位点的预突变光产物是含胞嘧啶的二聚体。通过使用313纳米光以及0.0%、0.1%或0.2%的苯乙酮来改变胸腺嘧啶-胸腺嘧啶二聚体与含胞嘧啶二聚体的比例，在此条件下分析了特定的突变频率响应。在2类抑制突变产生过程中观察到的变化与该模型一致，这表明导致2类抑制突变的G X C到A X T的转变是由突变位点处含胞嘧啶的嘧啶二聚体作为靶点的。
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Targeted mutation at cytosine-containing pyrimidine dimers: studies of Escherichia coli B/r with acetophenone and 313-nm light.含胞嘧啶嘧啶二聚体的靶向突变：用苯乙酮和313纳米光对大肠杆菌B/r的研究
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本文引用的文献
1
Absorption effects in volume irradiation of microorganisms.微生物体照射中的吸收效应。
Science. 1950 Mar 3;111(2879):229. doi: 10.1126/science.111.2879.229-a.
2
Mutational specificity of UV light in Escherichia coli: indications for a role of DNA secondary structure.紫外线在大肠杆菌中的突变特异性：DNA二级结构作用的迹象
Proc Natl Acad Sci U S A. 1982 Jul;79(13):4123-7. doi: 10.1073/pnas.79.13.4123.
3
The SOS regulatory system of Escherichia coli.大肠杆菌的SOS调控系统。
Cell. 1982 May;29(1):11-22. doi: 10.1016/0092-8674(82)90085-x.
4
UV-induced mutation hotspots occur at DNA damage hotspots.紫外线诱导的突变热点出现在DNA损伤热点处。
Nature. 1982 Jul 8;298(5870):189-92. doi: 10.1038/298189a0.
5
Specificity of UV mutagenesis in the lac promoter of M13lac hybrid phage DNA.M13lac杂交噬菌体DNA的乳糖操纵子启动子中紫外线诱变的特异性
Nature. 1982 Jun 17;297(5867):596-8. doi: 10.1038/297596a0.
6
Pyrimidine dimers induced in Escherichia coli DNA by ultraviolet radiation present in sunlight.阳光中的紫外线在大肠杆菌DNA中诱导形成嘧啶二聚体。
Photochem Photobiol. 1981 Oct;34(4):465-9.
7
Thermal resistance to photoreactivation of specific mutations potentiated in E. coli B/r ung by ultraviolet light.大肠杆菌B/r ung中经紫外线增强的特定突变对光复活的热抗性。
Mol Gen Genet. 1981;182(1):7-11. doi: 10.1007/BF00422759.
8
Crowding depression of UV-mutagenesis in E. coli.大肠杆菌中紫外线诱变的群体抑制作用
Mutat Res. 1980 Nov;73(1):43-58. doi: 10.1016/0027-5107(80)90134-7.
9
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Photochem Photobiol. 1980 Jun;31(6):571-7. doi: 10.1111/j.1751-1097.1980.tb03748.x.
10
Analysis of ultraviolet light-induced suppressor mutations in the strain of Escherichia coli K-12 AB1157: an implication for molecular mechanisms of UV mutagenesis.大肠杆菌K-12 AB1157菌株中紫外线诱导的抑制突变分析：对紫外线诱变分子机制的启示
Mol Gen Genet. 1980;180(2):283-91. doi: 10.1007/BF00425840.
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