Frankenhuis M T, Kramer M F, de Rooij D G
Vet Q. 1982 Apr;4(2):57-61. doi: 10.1080/01652176.1982.9693840.
In order to study kinetics of the spermatogenic epithelium in the adult boar, a method has been developed to prepare seminiferous tubules mounted as a whole, after which these tubules have been processed and studied as described previously only for rats, mice and hamsters. The tubules were fixed in Zenker for 24 hours and stained with periodic acid - Schiff - haematoxylin to identify the steps in spermatid development, or by Harris' hemalum to identify spermatogonia and spermatogonial divisions. Spermatid development in the boar is comparable with spermatid development in small laboratory rodents. The spermatogenic cycle was divided into 12 steps, each characterized by one step in the development of the acrosome or the shape of the nucleus of the spermatids. By studying whole mounts we could distinguish four classes of spermatogonia in the boar: undifferentiated A spermatogonia (A s, A pr, A al), differentiating A spermatogonia (A1, A2, A3, A4), intermediate and B spermatogonia. The boar demonstrates a striking similarity with small laboratory rodents.
为了研究成年公猪生精上皮的动力学，已开发出一种制备完整安装的生精小管的方法，之后按照之前仅针对大鼠、小鼠和仓鼠所描述的方法对这些小管进行处理和研究。小管在Zenker固定液中固定24小时，并用高碘酸-席夫-苏木精染色以识别精子细胞发育的阶段，或用哈里斯苏木精染色以识别精原细胞和精原细胞分裂。公猪的精子细胞发育与小型实验啮齿动物的精子细胞发育相当。生精周期分为12个阶段，每个阶段的特征是顶体发育的一个阶段或精子细胞核的形状。通过研究整装标本，我们可以在公猪中区分出四类精原细胞：未分化的A型精原细胞（As、Apr、Aal）、分化中的A型精原细胞（A1、A2、A3、A4）、中间型和B型精原细胞。公猪与小型实验啮齿动物表现出惊人的相似性。
